The move sets up a court battle between Brussels and the internet giant that could take years to resolve and make already tense relations between Europe and the U.S. tech giant even more fraught.
Google had been given 90 days to stop the favouritism or face a penalty of up to 5% of the average daily turnover of its parent company Alphabet.
Last week, EU officials said a plan that Google recently filed to comply with European regulations appeared to be a step "in the right direction". Google had initially said that they "respectfully" disagrees with the legal argument.
Brussels accuses Google of giving its own service too much priority in search results to the detriment of other price comparison services, such as TripAdvisor and Expedia.
Mexico quake death toll rises to at least 66
The epicenter of Thursday's quake was in the Pacific Ocean, about 100 kilometers (62 miles) off the town of Tonala in Chiapas. About half of the city hall collapsed in a pile of rubble, and streets were littered with the debris of ruined houses.
US Open: Rafael Nadal beats Juan Martin del Potro in semis
After dropping their respective first sets, both 31-year-olds found their game to prevail in four-set semifinals to reach the U.S. In the women's draw, Sloane Stephens , 24, and Madison Keys , 22, emerged from the first all-American semifinal in a U.S.
Manson Family Member Leslie Approved For Parole, 48 Years After Brutal Murders
Almost five decades ago, she said she held Rosemary down with a pillowcase over her head as others stabbed her dozens of times. Susan Atkins, (left) Patricia Krenwinkel (center) and Leslie Van Houten (right) were all members of the "Manson Family".
European Union competition commissioner Margrethe Vestager is investigating Google in three cases, as well as tech giants Apple, Facebook and Qualcomm.
Google has made a decision to appeal the record-breaking fine imposed on it by the European Union's highest antitrust authority in July.
While Google has declined to comment on the details of its appeal, the European Commission has said that it will defend its ruling in court.
Google is fighting its record $2.7 billion antitrust fine from the European Commission.
The EU is also investigating whether Google tried to squeeze out its rivals in online search advertising and through its Android mobile operating system.
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